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Determination of mimosine and 3,4-dihydroxypyridine in milk and plasma of goats

R. Puchala, J. J. Davis and T. Sahlu

Journal of Chromatography Biomedical Applications 685:375-378. 1996.

A simple method for determination of mimosine and 3,4-dihydroxypyridine(3,4-DHP)in plasma and milk was developed. Milk and plasma, with tyrosine as internal standard, were deproteinized using 9% trichloracetic acid and extracted with diethyl ether. Metabolites were separated by isocratic high-performance liquid chromatography, with 0.02 M ortho phosphoric acid (pH 2.5) at 0.5 ml/min and a Hypersil ODS microbore column. Mimosine, 3,4-DHP and tyrosine were detected at 275 nm. The recovery of the mimosine added to the plasma samples was 101.62.3% and 103.31.0% for milk samples. 3,4-DHP recovery for plasma samples was 101.20.9% and for milk samples 100.81.4%. The analyses yielded relative standard deviation of 2.65 and 2.82%, respectively.


 

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